Экстракция липидов из тканей животных

Существует множество методов количественного выделения интактных липидов из животных тканей. Наиболее удобным и точным, по нашему мнению, является способ, предложенный Моррисом Кейтсом в 1986 году: ...The procedure for small scale extraction of animal tissue, e.g. rat liver, is as follows: to 6 g of fresh tissue is added 3 ml of water and 30 ml of methanol-chloroform (2:1, v/v), and the mixture is blended in a Lourdes homogenizer or a Sorvall Omnimixer (60 ml capacity) for 2 min at room temperature. A Potter-Elvehjem glass homogenizer may also be used, but the tissue is blended first in the water before adding the organic solvents. The homogenate is centrifuged, the supernatant is decanted, and the residue is re-extracted with 38 ml of methanol-chloroform-0.2 N HCl (2:1:0.8, v/v) by homogenization for 2 min, to ensure extraction of phosphoinositides. After centrifugation, the combined supernatants are dilured with 20 ml each of chloroform and water and the phases separated by centrifugation or in a separatory funnel. The lower chloroform phase is withdrawn, neutralized by dropwise addition of 0.2 N methanolic NH4OH and concentrated in a rotary evaporator at 30-35oC (about 10 ml of benzene is added to aid in removal of traces of water), and the residue is dissolved to a suitable volume (e.g., 10 ml) in chloroform-methanol (2:1, v/v) and stored at -10oC... Morris Kates Techniques of lipidology. Isolation, analisys and identification of lipids// From: laboratory techniques in biochemistry and molecular biology, Vol. 3, part 2, Elsevier, 1986, P. 103. Если все эти несложные процедуры отразить в рисунке, то получится вот такая симпатичная схемка, которую можно распечатать и повесить на стенку в лаборатории...